Wavelength range: A fluorometer can typically measure fluorescence intensity at just one or a few discrete wavelengths. A fluorescence spectrophotometer can measure fluorescence intensity over a wide range of wavelengths, allowing for fluorescence emission spectra to be obtained.
Sensitivity: In general, fluorescence spectrophotometers are more sensitive than fluorometers as they can detect lower levels of fluorescence intensity.
Quantitative analysis: Fluorometers give a quantitative fluorescence intensity reading, while fluorescence spectrophotometers can quantitatively analyze fluorescence intensity as well as fluorescence spectra. This allows fluorescence spectrophotometers to separate and quantify different fluorescing analytes.
Spectral resolution: A fluorometer does not provide high spectral resolution, while a fluorescence spectrophotometer can measure fluorescence intensity with high precision at different wavelengths, providing more spectral resolution.
Applications: Fluorometers are generally used for more basic fluorescence measurements like assays, while fluorescence spectrophotometers enable applications like fluorescence spectroscopy, fluorescent labelling analysis, and identification of fluorescently tagged molecules based on their spectra.
So in summary, fluorescence spectrophotometers provide more detailed fluorescence data through measurement of spectra over a wide wavelength range, enabling applications like identification and quantitative analysis of multiple fluorescing components.

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